(Abstract)
Adipose tissue-derived stem cells (ASCs) is known to have the potential into many kinds of functional cells. However, there are few studies about Cl- channels of rabbit ASCs.
In this study, We isolated adipose tissue from rabbits and confirmed gene expressions of ASCs markers (CD29, CD90) and adipocyte markers with RT-PCR.
Further, we visualized and quantitated “lipid droplets (LDs)” in differentiated cells with fluorescent dyes, to evaluate this adipogenic differentiation. Large lipid droplets were found and cell proliferation was suppressed with adipogenic medium. Cl- channel blockers (NPPB and DIDS) suppressed the cell proliferation. However, Cl- channel blocker treatment inhibited LDs accumulation significantly in dose-dependent manner with adipogenic medium.
NPPB treatment diminished mitochondrial membrane potential and enhanced intracellular H2O2 production. This treatment suppressed LDs accumulation. However, IAA-94, that is known as CLIC channel blocker has not suppressed it.
These results imply Cl- channel play a important roles in adipogenic differentiation of rabbit ASCs.
